VMM - Vessel Motility Map

Load a multipage TIFF (one page = one frame)

Load TIFF

Optical flow (recompute after changes)

Downscale

Grid size (px)

Smoothing window (px)

Signal threshold (%)

Subtract static background

Voting (live)

Min. magnitude

Min. event duration

Direction tolerance

Min. votes

Direction as axis (±180°)

Map

Metric

Opacity

Directions

Background (max projection)

Direction: 0° = →, 90° = ↓. Magnitudes in px/frame.

VMM 1.0

Developed by dp-lab.info
This tool computes motility heatmap based on pyramidal Optical Flow and Voting. Its main application is for epifluorescence images capturing circulating cells in blood and lymphatic vessels.

dominant voteother directiondiscarded / below threshold